10112301
P4E6 cell line
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About This Item
UNSPSC Code:
41106514
Productos recomendados
biological source
human prostate
Quality Level
growth mode
Not specified
karyotype
Not specified
morphology
Epithelial
products
Stains positive for pancytokeratin, cytokeratin 8, vimentin (weak) and chromogranin A. Prostate markers expressed include prostate specific antigen (PSA) prostate specific membrane antigen (PSMA), negative for expression of androgen receptor (AR).
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Categorías relacionadas
Cell Line Origin
Prostate cancer well differentiated, early stage
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 7,8
THO1: 7,9.3
TPOX: 8
vWA: 17,19
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 7,8
THO1: 7,9.3
TPOX: 8
vWA: 17,19
Culture Medium
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. Additionally, any recipient wanting to use the cell line in the field of vaccines for the treatment or prevention of prostate cancer should first obtain permission from GemVax & KAEL Co., Ltd. Contact : Dr. Eujin Hyun, Head of R&D Department (eujinhyun@kaelgemvax.com).
Storage Class
6.2 - Infectious substances
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
N J Maitland et al.
Radiation research, 155(1 Pt 2), 133-142 (2000-12-20)
In Vitro Models to Study Cellular Differentiation and Function in Human Prostate Cancers. To augment the currently available models of human prostate cancer in vitro, we have established extended life-span epithelial cultures from biopsies of well-differentiated prostate cancers. The genetic
Shona H Lang et al.
The Prostate, 52(4), 253-263 (2002-09-05)
The metastatic potential of a series of prostate cell lines was analysed by measuring motility and invasiveness, and further correlated to the expression of epithelial differentiation markers. Invasion and motility were measured using in vitro assays. Immunohistochemistry of cell lines
Shona H Lang et al.
In vitro cellular & developmental biology. Animal, 42(8-9), 273-280 (2006-12-14)
Three-dimensional epithelial culture models are widely used to emulate a more physiologically relevant microenvironment for the study of genes and signaling pathways. Prostate epithelial cells can grow into solid cell masses or acinus-like spheroids in Matrigel. To test if the
Immortalization of human prostate cells with the human papillomavirus type 16 E6 gene.
Norman J Maitland et al.
Methods in molecular medicine, 88, 275-285 (2003-11-25)
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