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Merck

T8533

Sigma-Aldrich

o-Tolidine

≥95% purity, powder or chunks

Sinónimos:

3,3′-Dimethylbenzidine, 4,4′-Bianisidine

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About This Item

Fórmula lineal:
[-C6H3(CH3)-4-NH2]2
Número de CAS:
Peso molecular:
212.29
Beilstein/REAXYS Number:
2210640
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

o-Tolidine, ≥95%

Quality Level

assay

≥95%

form

powder or chunks

color

white to light brown

mp

128-131 °C (lit.)

solubility

water: slightly soluble 1.3 g/L at 25 °C

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(ccc1N)-c2ccc(N)c(C)c2

InChI

1S/C14H16N2/c1-9-7-11(3-5-13(9)15)12-4-6-14(16)10(2)8-12/h3-8H,15-16H2,1-2H3

InChI key

NUIURNJTPRWVAP-UHFFFAOYSA-N

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General description

o-Tolidine is a diacidic dye which is sparingly soluble in water and easily soluble in ether and alcohol. On exposure to chlorine or bromine vapors, it forms an intense blue color. This forms the basis for detection of chlorine, nitrates and hydrogen peroxide in air or water. It turns yellow on reacting with free chlorine. o-Tolidine easily forms acid salts.

Application

o-Tolidine has also been used to determine the presence of laccase activity in T. versicolor fungal colonies.
Reactivo colorimétrico sensible para oro y cloro libre en agua.

Warning

Puede ser cancerígeno.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

212.0 °F - closed cup

flash_point_c

100 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Wiley-VCH
Ullmann's Fine Chemicals null
Purification and Characterization of Laccase from the
White Rot Fungus Trametes versicolor
Moon-Jeong
Journal of Microbiology (2005)
Leo M.L. Nollet
Handbook of Water Analysis (2013)
S P Tuffli et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 13(2), 177-179 (2001-04-06)
Eight different diets were each fed to 6 cats to evaluate the effect on a guaiac and an o-tolidine fecal occult blood test. Fecal samples were collected from day 5 through day 7. Canine blood or pure cottage cheese were
T Platzek et al.
Human & experimental toxicology, 18(9), 552-559 (1999-10-19)
Azo dyes represent the major class of dyestuffs. They are metabolised to the corresponding amines by liver enzymes and the intestinal microflora following incorporation by both experimental animals and humans. For safety evaluation of the dermal exposure of consumers to

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