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B8806

Sigma-Aldrich

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester

1 mg/mL in DMSO

Sinónimos:

BCECF-AM solution, Spiro(isobenzofuran-1(3H),9′-(9H)xanthene)-2′,7′-dipropanoic acid

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About This Item

EC Number:
200-664-3
MDL number:
MFCD00036969
UNSPSC Code:
12171500
NACRES:
NA.47

description

Mixture of six isomeric compounds

Quality Level

200

form

liquid

concentration

1 mg/mL in DMSO

color

colorless to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O

InChI

1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3

InChI key

NTECHUXHORNEGZ-UHFFFAOYSA-N

Application

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester has been used for the estimation of vacuolar pH and intracellular pH.

Biochem/physiol Actions

An intracellular fluorescent pH indicator.
Hydrolyzed to BCECF by cytosolic esterases.

Physical form

Solution in 0.1% BCECF AM Ester and 99.9% DMSO

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

185.0 °F

flash_point_c

85 °C

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Certificados de análisis (COA)

Lot/Batch Number
SLCD5545
SLBZ8252
SLBX8427
SLBS6911
SLBP2622V

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A K Ho et al.
The American journal of physiology, 261(4 Pt 1), C642-C649 (1991-10-01)
In rat pinealocytes, activation of alpha 1-adrenergic receptors leads to increases in intracellular pH (pHi). In this study, the role of pHi on adrenergic regulation of cyclic nucleotide accumulation was investigated using ammonium chloride, which increased pHi, and sodium propionate
Salil Chanroj et al.
The Journal of biological chemistry, 286(39), 33931-33941 (2011-07-29)
The complexity of intracellular compartments in eukaryotic cells evolved to provide distinct environments to regulate processes necessary for cell proliferation and survival. A large family of predicted cation/proton exchangers (CHX), represented by 28 genes in Arabidopsis thaliana, are associated with
Chengzhi Hu et al.
Lab on a chip, 17(4), 671-680 (2017-01-18)
Pollen tubes are tip-growing plant cells that deliver the sperm cells to the ovules for double fertilization of the egg cell and the endosperm. Various directional cues can trigger the reorientation of pollen tube growth direction on their passage through
Viraj P Nirwan et al.
Nanomaterials (Basel, Switzerland), 11(2) (2021-03-07)
Herein, we report the fabrication and characterization of novel polycaprolactone (PCL)-based nanofibers functionalized with bare (ligand-free) titanium nitride (TiN) nanoparticles (NPs) for tissue engineering applications. Nanofibers were prepared by a newly developed protocol based on the electrospinning of PCL solutions
Evaluation of intracellular pH regulation and alkalosis defense mechanisms in preimplantation embryos.
Dagilgan S, et al.
Theriogenology, 83, 1075-1084 (2015)
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