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Merck

B3426

Millipore

Bile salts mixture

suitable for microbiology

Sinónimos:

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

packaging

pkg of 100 g

loss

≤6% loss on drying

color

yellow to brownish-yellow

pH

7±0.5

solubility

distilled water: freely soluble

application(s)

microbiology

storage temp.

10-30°C

General description

Bile salts mixture is a combination of an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Bile salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic, and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that inhibits Gram-positive bacteria and induced germination. It also inhibits the outgrowth of the germinated spores but does not interfere with the growth of Enterobacteriaceae.

Application

Bile salts mixture is recommended in diverse media like MacConkey Agar, SS Agar, and Violet Red Bile Agar. It increases the selectivity of the media to Enterobacteriaceae by inhibiting Gram-positive bacteria. It is used as a selective agent in culture media for the selective growth of intestinal bacteria like E. coli, other fecal coliforms, and fecal Streptococci. But it is also used as a selective agent in media for the detection of Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species.

Preparation Note

Extracted from purified, fresh bile.

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Chhatra B Kunwar et al.
Microbiology (Reading, England), 166(12), 1149-1159 (2020-11-19)
Colonization of the gastrointestinal tract by enterohaemorrhagic Escherichia coli (EHEC) is critically dependent on its ability to sense and respond to various microenvironments within the host. EHEC exposure to physiologically relevant levels of bile salts upregulates the two-component system, pmrAB

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