11427857910
Roche
Fluorescein-12-UTP
≥85% (HPLC), solution
Sinónimos:
Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
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About This Item
Fórmula empírica (notación de Hill):
C39H41N4O22P3
Número de CAS:
Peso molecular:
1010.68
UNSPSC Code:
41116100
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Quality Level
assay
≥85% (HPLC)
form
solution
mol wt
1034.4
packaging
pkg of 25 μL (250 nmol; 10 mM)
manufacturer/tradename
Roche
storage temp.
−20°C
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General description
Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C39H37N4O22P3Li4.
Application
Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.
Other Notes
For life science research only. Not for use in diagnostic procedures.
For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
does not flash
flash_point_c
does not flash
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