SCT212

HaloTag AcidiFluor Orange Ligand

Live Cell imaging probe for acidic pH. It shows weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH.

• UNSPSC Code: 12352207
• NACRES: NA.71

Propiedades

• form: lyophilized
• detection method: fluorometric

Descripción

General description

HaloTag AcidiFluor ORANGE is an acidic pH indicator. It consists of AcidiFluor ORANGE dye fused with HaloTag ligand, which forms a covalent bond with HaloTag. It shows quite weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH. Since it is cell impermeable reagent, it can be used to label membrane-associated HaloTag fused proteins just by mixing and then monitoring pH changes. Relatively photostable fluorescence of AcidiFluor ORANGE enables time lapseimaging of pH change and tracking of endocytic/exocytic vesicles for long-time period.

Spectral Properties:
Absorbance (max): 520nm
Emission (max): 565nm

Research Category:
Live Cell Dye Signaling
Neuroscience.

HaloTag is a registered tradeMark of Promega Corporation.
AcidiFluor is a trademark of Goryo Chemical Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial