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SCM075

Sigma-Aldrich

Inhibidor ROCK (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Sinónimos:

Y-27632

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

100

form

liquid

mol wt

320.26 g/mol

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

General description

El inhibidor ROCK (Y-27632) es un inhibidor competitivo del ATP selectivo de la proteína cinasa asociada a Rho (ROCK) que incluye p160Rock (Ki=140 nM) y Rock-II También actúa como un potente inhibidor de la sensibilización al Ca2+ inducida por agonista de la fosforilación de miosina y la contracción del músculo liso. El tratamiento de una hora con Y-27632 10uM bloquea la apoptosis de las células madre embrionarias humanas (hESCs) cultivadas disociadas, aumenta la supervivencia y la eficacia de clonación de las hESCs sin afectar a su pluripotencia1. Recientemente algunos informes demostraron que la aplicación de Y-27632 y Accutase (Nº de ref. SCR005), una mezcla formulada de enzimas digestivas, mejora la tasa de supervivencia pos-descongelamiento de las hESC individuales utilizando métodos convencionales de congelación lenta y descongelación rápida

Storage and Stability

Antes de la reconstitución: Mantener a -20ºC, protegido de la luz durante 2 años. Después de la reconstitución: 1 años a -20ºC, protegido de la luz

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Katarzyna Pańczyk et al.
MedChemComm, 9(11), 1933-1948 (2018-12-21)
A series of 17 new phenoxyacetamides has been prepared via multistep chemical synthesis as a continuation of the research carried out by our group on di- and tri-substituted phenoxyalkyl and phenoxyacetyl derivatives of amines. The obtained compounds vary in an
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Xin Geng et al.
JCI insight, 5(14) (2020-06-17)
During the growth of lymphatic vessels (lymphangiogenesis), lymphatic endothelial cells (LECs) at the growing front sprout by forming filopodia. Those tip cells are not exposed to circulating lymph, as they are not lumenized. In contrast, LECs that trail the growing
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
Xin Lin et al.
EBioMedicine, 69, 103463-103463 (2021-07-06)
Family with Sequence Similarity 13, Member A (FAM13A) gene has been consistently associated with COPD by Genome-wide association studies (GWAS). Our previous study demonstrated that FAM13A was mainly expressed in the lung epithelial progenitors including Club cells and alveolar type
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