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Key Documents

SCM008

Sigma-Aldrich

Mouse Neural Stem Cell Expansion Medium

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About This Item

UNSPSC Code:
41106200
eCl@ss:
32160801
NACRES:
NA.32

description

Neural Stem Cells

Quality Level

type

for cell culture

form

liquid

technique(s)

cell culture | stem cell: suitable

General description

The Mouse Neural Stem Cell Expansion Medium allows for the growth and proliferation of mouse neural stem cells. This product includes the Neural Stem Cell Basal Medium and the supplements bFGF, EGF, and Heparin. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells.

Components

500 mL of Neural Stem Cell Basal Medium (Cat.# SCM003) bFGF, 10 ug EGF, 10 ug Heparin, 1.5 mg

Quality

Each lot of medium is tested for the optimal cell growth, proliferation and differentiation of mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4

Storage and Stability

Part number SCM003, the Neural Stem Cell Basal Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. bFGF, EGF and heparin solutions should be stored in working aliquots at -20°C for up to six months. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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