QIA58
BrdU Cell Proliferation Assay
This proliferation assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells.
Sinónimos:
Bromodeoxyuridine Assay
About This Item
Productos recomendados
Quality Level
usage
sufficient for 1000 tests
sufficient for 200 tests
packaging
pkg of 1 96-well plate(s)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
input
sample type intact cells
sample type intact cells
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
Components
Warning
Specifications
Principle
Storage and Stability
Prior to use:
1. Remove the Fixative/Denaturing Solution and place at room temperature for at least 4 h prior to use. Precipitates that may occur while cold should go back into solution.
2. Reconstitute the Peroxidase Goat Anti-Mouse IgG with the appropriate volume of 1X PBS.
• For the 200 test kit, reconstitute Peroxidase Goat Anti-Mouse IgG in 125 µl of 1X PBS. Use Conjugate Diluent for any further lot-specific dilution.
• For the 1000 test kit, reconstitute Peroxidase Goat Anti-Mouse IgG in 250 µl of 1X PBS. Use Conjugate Diluent for any further lot-specific dilution.
Allow solution to stand at room temperature for 10 min. (or until solution is clear). Divide into small aliquots; aliquots not to be used that same day should be stored at -20°C in a non-frostfree freezer.
Once the kit components have been thawed for use:
• The BrdU label and 100X Anti-BrdU Antibody should be divided into small aliquots and stored at -20°C in a non-frostfree freezer with the Peroxidase Goat Anti-Mouse IgG; avoid multiple freeze/thaw cycles.
• All the other kit components (Substrate, Antibody Diluent, Conjugate Diluent, 20X Plate Wash Concentrate, Fixative/Denaturing Solution, Stop Solution) should be stored at 4°C.
Other Notes
Muir, D., et al. 1990. Analytical Biochemistry185, 377.
Lanier, T. L., et al. 1989. Carcinogenesis10, 1341.
Magaud, J.-P., et al. 1988. J. Immunol. Methods106, 95.
Collins, S.J. 1987. Blood70, 1233.
Porstmann, T., et al. 1985. J. Immunol. Methods82, 169.
Raza, A., et al. 1985. Cytometry6, 633.
Morstyn, G., et al. 1983. J. Clin. Invest.72, 1844.
Gratzner, H.G. 1982. Science218, 474.
Legal Information
signalword
Danger
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
3 - Flammable liquids
flash_point_f
69.8 °F
flash_point_c
21 °C
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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