PMEF-N
EmbryoMax® Primary Mouse Embryonic Fibroblasts
Sinónimos:
NEO-MEFs, Neo MEF Feeder Cells, Neo Mouse Feeder Cells, Neomycin Restistant PMEFs
About This Item
Productos recomendados
biological source
mouse
Quality Level
manufacturer/tradename
Specialty Media
EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type: mouse embryonic stem cell(s)
shipped in
liquid nitrogen
General description
Plating MEF Feeder Cells
Procedure:
1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
Cell Line Description
Packaging
Storage and Stability
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificados de análisis (COA)
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Protocolos
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
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