MABF2815
Anti-SARS-CoV-2 Spike RBD Antibody, clone 6C2-G9
About This Item
Productos recomendados
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
6C2-G9, monoclonal
mol wt
calculated mol wt 141.18 kDa
observed mol wt ~200 kDa
species reactivity
virus
packaging
antibody small pack of 100 μg
technique(s)
ELISA: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
-10 to -25°C
target post-translational modification
unmodified
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from HEK293T cells expressing full-length spike protein of SARS-CoV-2 virus.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Spike glycoprotein in HEK293T cells expressing full-length spike protein of SARS-CoV-2, but not in lysate from wild-type HEK293T cells.
Tested Applications
Neutralizing: A representative lot neutralized SARS-CoV-2 Spike protein RBD to ACE2 in Neutralizing applications (Courtesy of Kento Abe and Anne-Claude Gingras, Toronto).
ELISA Analysis: A 1:3 dilution from a representative lot detected SARS-CoV-2 Spike protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
Western Blotting Analysis: A 1:400 dilution from a representative lot detected Sars-CoV-2 Spike protein in Whole cell lysates of HEK293T cells expressing full-length spike protein of SARS-CoV-2. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
Neutralizing: A representative lot neutralized SARS-CoV-2 Spike protein RBD binding to ACE2 receptor. (Abe, K.T., et al. (2020). JCI Insight 5(19):e142362).
Immunoprecipitation Analysis: A representative lot immunoprecipitated SARS-CoV-2 Spike protein. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Storage and Stability
Other Notes
Disclaimer
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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