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Key Documents

MAB333

Sigma-Aldrich

Anti-Synaptobrevin Antibody, clone SP10

ascites fluid, clone SP10, Chemicon®

Sinónimos:

VAMP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SP10, monoclonal

species reactivity

rat, hamster, salamander, pig, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VAMP1(6843)

Specificity

Synaptobrevin [VAMP]. MAB333 reacts with VAMP-1 and VAMP-2 fusion protein expressed in bacterial system.

Immunogen

Crude synaptic immunoprecipitate (Human).

Application

Anti-Synaptobrevin Antibody, clone SP10 is an antibody against Synaptobrevin for use in WB, IH.
Immunohistochemistry at >1:25.

Western blotting at 1:100-1:10,000.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Linkage

Replaces: AB5863P

Physical form

Liquid, contains sodium azide.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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S E Bradford et al.
Neuroscience, 128(4), 751-765 (2004-10-07)
Certain excitatory pathways in the rat hippocampus can release aspartate along with glutamate. This study utilized rat hippocampal synaptosomes to characterize the mechanism of aspartate release and to compare it with glutamate release. Releases of aspartate and glutamate from the
G Bonanno et al.
British journal of pharmacology, 129(8), 1780-1786 (2000-04-26)
The release of [(3)H]-dopamine ([(3)H]-DA) from human neocortex nerve terminals was studied in synaptosomes prepared from brain specimens removed in neurosurgery and exposed during superfusion to different releasing stimuli. Treatment with 15 mM KCl, 100 microM 4-aminopyridine, 1 microM ionomycin
D M Sherry et al.
The Journal of comparative neurology, 431(4), 424-436 (2001-02-27)
Vesicle associated membrane protein (VAMP; also known as synaptobrevin) is a key component of the core complex needed for docking and fusion of synaptic vesicles with the presynaptic plasma membrane. Recent work indicates that the precise complement of presynaptic proteins
Peter Nagele et al.
Anesthesiology, 103(4), 768-778 (2005-09-30)
Volatile general anesthetics (VAs) have a number of synaptic actions, one of which is to inhibit excitatory neurotransmitter release; however, no presynaptic VA binding proteins have been identified. Genetic data in Caenorhabditis elegans have led to the hypothesis that a
S E Ahmari et al.
Nature neuroscience, 3(5), 445-451 (2000-04-19)
Little is known about presynaptic assembly during central nervous system synaptogenesis. Here we used time-lapse fluorescence imaging, immunocytochemistry and electron microscopy to study hippocampal neuronal cultures transfected with a fusion construct of the presynaptic vesicle protein VAMP and green fluorescent

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