MAB3101
Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 5B9.2
clone 5B9.2, Chemicon®, from mouse
Sinónimos:
Cx45
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
5B9.2, monoclonal
species reactivity
rat, rabbit, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GJC1(10052)
rat ... Gjc1(266706)
Specificity
Recognizes Human Connexin 45.
SPECIES REACTIVITIES:
Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.
SPECIES REACTIVITIES:
Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.
Immunogen
Epitope: near C-terminus, cytoplasmic
The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.
Application
Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 5B9.2 detects level of Connexin 45 & has been published & validated for use in IC & WB.
Immunblotting (~46 kDa)
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Physical form
Format: Purified
Liquid at 1 mg/mL in 0.02M PB with 0.25 M NaCl, pH=7.6 containing 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Expression of connexins 36, 43, and 45 during postnatal development of the mouse retina.
Alexandre Hiroaki Kihara, Leandro Mantovani de Castro, Monica Aparecida Belmonte et al.
Journal of Neurobiology null
Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bihomotypic gap junctions, with scaffolding contributed by zonula occludens-1.
Li, X; Kamasawa, N; Ciolofan, C; Olson, CO; Lu, S; Davidson, KG; Yasumura, T; Shigemoto et al.
The Journal of Neuroscience null
Axonal synapses utilize multiple synaptic ribbons in the mammalian retina.
Kim, HL; Jeon, JH; Koo, TH; Lee, UY; Jeong, E; Chun, MH; Moon, JI; Massey, SC; Kim, IB
Testing null
Electrical synapses in retinal ON cone bipolar cells: subtype-specific expression of connexins.
Han, Y; Massey, SC
Proceedings of the National Academy of Sciences of the USA null
Alexandre H Kihara et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 28(1), 39-52 (2009-10-06)
Connexin (Cx) channels and hemichannels are involved in essential processes during nervous system development such as apoptosis, propagation of spontaneous activity and interkinetic nuclear movement. In the first part of this study, we extensively characterized Cx gene and protein expression
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