GCYT1-16K
MILLIPLEX® Chicken Cytokine/Chemokine Panel 1 - Immunology Multiplex Assay
About This Item
Productos recomendados
species reactivity
chicken
manufacturer/tradename
Milliplex®
assay range
accuracy: 87-100%
(using the overnight protocol)
accuracy: 95-100%
(using the 2 hour protocol)
standard curve range: 12.21-20,000 pg/mL
(for RANTES)
standard curve range: 122.1-500,000 pg/mL
(for IL-6)
standard curve range: 18.3-75,000 pg/mL
(for IFNγ)
standard curve range: 18.3-75,000 pg/mL
(for MIP-3α)
standard curve range: 24.4-100,000 pg/mL
(for IL-10)
standard curve range: 3.66-15,000 pg/mL
(for IFNα)
standard curve range: 36.6-150,000 pg/mL
(for IL-21)
standard curve range: 4.88-20,000 pg/mL
(for MIP-1β)
standard curve range: 4.88-20,000 pg/mL
(for VEGF)
standard curve range: 48.8-200,000 pg/mL
(for IL-2)
standard curve range: 7.32-30,000 pg/mL
(for IL-16)
standard curve range: 9.75-40,000 pg/mL
(for M-CSF)
inter-assay cv: <20%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP®)
shipped in
wet ice
storage temp.
2-8°C
General description
EMD Millipore′s MILLIPLEX® Chicken Cytokine/Chemokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in chicken serum, plasma and PBMC samples: Interferon alpha (IFNα), Interferon gamma (IFNγ), Interleukin 2 (IL-2), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Interleukin 16 (IL-16), Interleukin 21 (IL-21), Macrophage Inflammatory protein-1β (MIP-1b), Macrophage Inflammatory protein-3 (MIP-3a), RANTES (regulated on activation, normal T cell expressed and secreted), Macrophage colony-stimulating factor (M-CSF), Vascular endothelial growth factor (VEGF).
Note: The protocol recommends a 1:4 dilution of serum and plasma samples.
MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.
Each MILLIPLEX® panel and kit includes:
- Quality controls (QCs) to qualify assay performance
- Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
- Optimized serum matrix to mimic native analyte environment in serum and plasma samples
- Detection antibody cocktails designed to yield consistent analyte profiles within panel
The MILLIPLEX® Chicken Cytokine/Chemokine Panel thus enables you to focus on the therapeutic potential of cytokines and chemokines.
Specificity
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Application
- This assay may be run overnight (16-18 hours) at 2-8°C or for 2 hours at room temperature (20-25°C) with agitation on a plate shaker.
- A maximum of 25 μL per well 1:4 serum or plasma diluted in Serum Matrix should be used for all analytes. Tissue culture supernatants may require dilution in appropriate control medium. Tissue/cell extracts should be prepared in neutral buffers containing reagents and conditions that do not interfere with assay performance.
Analytes Available:
- IL-16
- IFN-α
- IFNγ
- IL-10
- IL-21
- IL-2
- M-CSF
- IL-6
- MIP-3α
- MIP-1β
- RANTES
- VEGF
Research Category:
- Inflammation & Immunology
Research Sub-Category:
- Inflammation & Autoimmune Mechanisms
- Inflammation
- Infectious Diseases
- Cytokines & Cytokine Receptors
- Oncology
- Obesity
- Metabolic Disorders
Features and Benefits
Packaging
Analysis Note
Legal Information
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
target_organs
Respiratory Tract
Storage Class
10 - Combustible liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Contenido relacionado
Learn the benefits of using biomarker detection multiplex immunoassays for laboratory, companion, and agricultural animal research and explore MILLIPLEX® multiplex assays for veterinary medicine and animal health research.
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