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Key Documents

AP510

Sigma-Aldrich

Sheep-Anti-Rabbit IgG secondary Antibody

2 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

sheep

Quality Level

antibody form

purified antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

concentration

2 mg/mL

technique(s)

ELISA: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.

Specificity

This antibody reacts with rabbit IgG and rabbit IgG (Fc) as demonstrated by ELISA. Minimal cross-reactivity detected with Human IgG and Rabbit IgM; little to no cross-reactivity detected with Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.

Immunogen

Rabbit IgG

Application

Research Category
Secondary & Control Antibodies
Research Sub Category
Isotype Control Antibodies
Sheep-anti-Rabbit IgG secondary antibody detects level of Rabbit IgG secondary & has been published & validated for use in ELISA.

Quality

Evaluated by ELISA against Human IgG, Mouse IgG, Rat IgG, Chicken IgM, Goat IgG, Guinea Pig IgG, Hamster IgG, Rabbit IgG, Rabbit IgG (Fc), and Rabbit IgM.

ELISA Analysis: A representative lot detected Sheep Anti-Human IgM, (Fc) in ELISA. (1:200 - 1,000 recommended dilution.)

Target description

150 kDa calculated

Physical form

Format: Purified
Octanoic Acid and Ammonium Sulfate cut. Depleted against Goat IgG and Rabbit IgM
Purified sheep polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human IgG, Mouse IgG, Rat IgG whole molecule, Chicken IgM whole molecule, Goat IgG whole molecule, Guinea Pig IgG, Hamster IgG whole molecule, Rabbit IgG, Rabbit IgG (Fc), and Rabbit IgM whole molecule

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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