Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

ABS562

Sigma-Aldrich

Anti-CH25H Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CH25H(9023)

General description

CH25H , also known as Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH, and encoded by the gene name CH25H, is a member of the sterol desaturase family. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol, which in turn leads to repression of the cholesterol biosynthetic enzymes. Recent studies have shown that CH25H may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. Other studies have also indicated that in testis, production of 25-hydroxycholesterol may play a role in Leydig cell differentiation.

Immunogen

Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to human CH25H near the N-terminus.

Application

Research Category
Signaling
Research Sub Category
Signaling Neuroscience
This Anti-CH25H antibody is validated for use in Western Blotting for the detection of CH25H.

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected CH25H in 10 µg of THP-1 cell lysate.

Target description

~32 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico