ABN1362
Anti-RBPMS Antibody
from rabbit, purified by affinity chromatography
Sinónimos:
RNA-binding protein with multiple splicing, RBPMS, RBP-MS, Heart and RRM expressed sequence, Hermes
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, rat
technique(s)
immunofluorescence: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RBPMS(11030)
General description
RNA-binding protein with multiple splicing (UniProt Q93062; also known as Heart and RRM expressed sequence, Hermes, RBP-MS) is encoded by the RBPMS (also known as HERMES) gene (Gene ID 11030) in human. RBPMS is a member of the RRM (RNA recognition motif) family of RNA-binding proteins. Transcriptome-wide study using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in HEK293 cells reveals both exonic mature and intronic pre-mRNA-binding sites, in agreement with the nuclear and cytoplasmic localization of the RRM family proteins. RBPMS is found to be exclusively expressed in retinal ganglion cells (RGCs) and displaced RGCs (dRGCs). RBPMS expression can therefore be utitized as a RGC marker, and the RBPMS gene promoter could be utilized for driving RGC-specific transgene expression.
Specificity
Expected to react with all five spliced isoforms (UniProt Q93062-1 through Q93062-5).
Immunogen
Epitope: N-terminal region.
KLH-conjugated linear peptide corresponding to a sequence from the N-terminal region of human RBPMS.
Application
Anti-RBPMS Antibody is an antibody against RBPMS for use in Western Blotting, Immunofluorescence.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Western Blotting Analysis: A representative lot detected endogenous RBPMS in mouse and rat retina extracts, as well as exogenously expressed human RBPMS in transfected HEK293T cells (Rodriguez, A.R., et al. (2014). J. Comp. Neurol. 522(6):1411-1443).
Immunofluorescence Analysis: A representative lot detected RBPMS immunoreactivity primarily associated with cell bodies located in the ganglion cell layer (GCL) of paraformaldehyde-fixed mouse retinas by fluorescent immunohistochemistry using whole-mounted retinas (Rodriguez, A.R., et al. (2014). J. Comp. Neurol. 522(6):1411-1443).
Immunofluorescence Analysis: An 1:5,000 dilution of a representative lot detected RBPMS immunoreactivity among cells in the ganglion cell layer (GCL) of paraformaldehyde-fixed mouse retinas by fluorescent immunohistochemistry (Courtesy of Dr. Nicholas Brecha, David Geffen School of Medicine at UCLA).
Immunofluorescence Analysis: A representative lot detected RBPMS immunoreactivity primarily associated with cell bodies located in the ganglion cell layer (GCL) of paraformaldehyde-fixed mouse retinas by fluorescent immunohistochemistry using whole-mounted retinas (Rodriguez, A.R., et al. (2014). J. Comp. Neurol. 522(6):1411-1443).
Immunofluorescence Analysis: An 1:5,000 dilution of a representative lot detected RBPMS immunoreactivity among cells in the ganglion cell layer (GCL) of paraformaldehyde-fixed mouse retinas by fluorescent immunohistochemistry (Courtesy of Dr. Nicholas Brecha, David Geffen School of Medicine at UCLA).
Quality
Evaluated by Western Blotting in mouse embryonic stem cell (mESC) lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected RBPMS in 10 µg of mouse embryonic stem cell (mESC) lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected RBPMS in 10 µg of mouse embryonic stem cell (mESC) lysate.
Target description
~25 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal antibody in PBS with 1% BSA and 0.09 % sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
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Frontiers in neuroscience, 13, 719-719 (2019-07-30)
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