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Merck
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Key Documents

AB9276

Sigma-Aldrich

Anti-Filamin B Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FLNB(2317)

Specificity

Filamin B

Immunogen

Synthetic peptide from human filamin B. The immunogen peptide corresponds to a sequence near the hinge 2 region of Filamin B (near C-term) and is not present in the two C-terminally truncated splice variants of Filamin B (Var-2 and Var-3). The peptide shows no significant similarity to other antigens in the human protein database including Filamin A or Filamin C.

Application

Anti-Filamin B Antibody detects level of Filamin B & has been published & validated for use in WB.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western blot: 0.1 μg/mL on HeLa cell lysate and LAN-1 human neuroblastoma cell lysate. 0.25-0.5 μg/mL on HuVec cell lysate. Reacts with the ~278 kDa Filamin B protein.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in 0.02 M PBS, 0.25 M NaCl containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Analysis Note

Control
HeLa cells

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Frances Willenbrock et al.
Biophysical journal, 105(5), 1110-1122 (2013-09-10)
Shear flow assays are used to mimic the influence of physiological shear force in diverse situations such as leukocyte rolling and arrest on the vasculature, capture of nanoparticles, and bacterial adhesion. Analysis of such assays usually involves manual counting, is
Vascular and connective tissue anomalies associated with X-linked periventricular heterotopia due to mutations in Filamin A.
Reinstein, E; Frentz, S; Morgan, T; Garcia-Mi?aur, S; Leventer, RJ; McGillivray et al.
European Journal of Human Genetics null
Ji Li et al.
eLife, 7 (2018-07-31)
Alternative splicing of mRNA precursors represents a key gene expression regulatory step and permits the generation of distinct protein products with diverse functions. In a genome-scale expression screen for inducers of the epithelial-to-mesenchymal transition (EMT), we found a striking enrichment

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