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AB3316P

Sigma-Aldrich

Anti-Monocarboxylate Transporter 4 Antibody

Chemicon®, from rabbit

Sinónimos:

MCT4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC16A3(9123)

Specificity

Recognizes human Monocarboxylate Transporter 4 (MCT4). The immunogen shows no significant sequence homology with other MCT. MCT4 is prominently expressed in skeletal muscle and other cells with high glycolytic rate such as tumors cells and white blood cells suggesting an important role in lactic acid efflux.

Note: According to new nomenclature, the old MCT3 has now been reclassified as MCT4. Consult Halestrap and Price (1999) Biochem. J. 343:281-299.

Immunogen

A 19 amino acid peptide sequence within the cytoplasmic domain #4 of human MCT4 (Wilson et al., 1998; Price et al., 1998).

Application

Anti-Monocarboxylate Transporter 4 Antibody detects level of Monocarboxylate Transporter 4 & has been published & validated for use in ELISA & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1-5 μg/mL using ECL.
Immunohistochemistry: Not tested. Recommended antibody testing dilution: 5-10 μg/mL on paraformaldehyde fixed tissue.
ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog number AG219) per well.
Optimal working dilutions must be determined by the end user.

Linkage

Replaces: AB3548P

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Cellular assessment of muscle in COPD: case studies of two males.
HJ Green, E Bombardier, ME Burnett, S Iqbal, KA Webb, J Ouyang
International Journal of General Medicine null
Monocarboxylate transporters, blood lactate removal after supramaximal exercise, and fatigue indexes in humans.
Thomas, C; Perrey, S; Lambert, K; Hugon, G; Mornet, D; Mercier, J
Journal of Applied Physiology (1985)
Targeting lactate-fueled respiration selectively kills hypoxic tumor cells in mice.
Sonveaux, Pierre, et al.
The Journal of Clinical Investigation (2008)
Céline Pinheiro et al.
European journal of cancer (Oxford, England : 1990), 45(13), 2418-2424 (2009-07-25)
The aim of the present work was to assess the role of monocarboxylate transporters (MCTs), namely MCT1 and MCT4 as well as MCT/CD147 co-expression in gastric tissues and evaluate their clinico-pathological significance in gastric carcinoma. For that, we analysed the
Stefan De Smet et al.
Frontiers in physiology, 8, 347-347 (2017-06-18)
In the setting of "living high," it is unclear whether high-intensity interval training (HIIT) should be performed "low" or "high" to stimulate muscular and performance adaptations. Therefore, 10 physically active males participated in a 5-week "live high-train low or high"

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