AB1587P
Anti-Adenosine A1 Receptor Antibody
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat, sheep, guinea pig, pig
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ADORA1(134)
Specificity
Recognizes Adenosine A1 receptor (A1R). Recognizes the 79 kDa and 39 kDa bands of in cortical membranes from rat, porcine and lamb. The 79 kDa band can be converted to 39 kDa band by agonist or antagonist treatment and could represent a A1R dimers.
Immunogen
Amino acid sequence corresponding to the 3rd extracellular domain (amino acids 163-176, GEPVIKCEFEKVIS) of the rat A1R gene.
Application
Anti-Adenosine A1 Receptor Antibody detects level of Adenosine A1 Receptor & has been published & validated for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen tissue sections. Light fixation is recommended. 2% 0.2% picric acid {Cooke, HJ (1999) AJP Gastrointest Liver Physiol 276:4516-4626}
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
Western blot: 1-10 μg/mL (Chemiluminescence technique): Membrane preparations are recommended for enhanced signals {Smith et al (2001) Biochem Pharmacology 61:237-244} Higher concentrations of the antibody may be necessary if calorimetric detection or milk-based antibody diluent are used.
ELISA: 1:10,000-1:50,000 (50-100 ng A1R peptide (Cat. Number AG289)/well)
Optimal working dilutions must be determined by end user.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
Western blot: 1-10 μg/mL (Chemiluminescence technique): Membrane preparations are recommended for enhanced signals {Smith et al (2001) Biochem Pharmacology 61:237-244} Higher concentrations of the antibody may be necessary if calorimetric detection or milk-based antibody diluent are used.
ELISA: 1:10,000-1:50,000 (50-100 ng A1R peptide (Cat. Number AG289)/well)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Target description
79 kDa and 39 kDa
Physical form
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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