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Key Documents

AB1528SP

Sigma-Aldrich

Anti-Nerve Growth Factor-β Antibody

Chemicon®, from sheep

Sinónimos:

NGFbeta

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

sheep

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

chicken, human, rat, mouse

should not react with

bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NGF(4803)

Specificity

Nerve growth factor-beta (NGF). By one site ELISA, less than 1% cross-reactivity against recombinant human BDNF or neurotrophin 4. Approximately 2% cross-reactivity against neurotrophin 3.

Immunogen

Mouse nerve growth factor-beta purified from salivary glands. Antigen purity greater than 95% by PAGE.

Application

Anti-Nerve Growth Factor-β Antibody is an antibody against Nerve Growth Factor-β for use in ELISA, WB, IH.
Immunohistochemistry: 1-10 μg/mL (see suggested protocol).

Immunoblotting: 1-10 μg/mL

ELISA: 1-10 μg/mL

Inhibition of biological activity in vitro: 1-10 μg/mL
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins

Linkage

Replaces: 04-1119

Physical form

Format: Purified
Protein G purified immunoglobulin. Lyophilized from PBS, no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to one year. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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The role of mesopontine NGF in sleep and wakefulness.
Ramos, OV; Torterolo, P; Lim, V; Chase, MH; Sampogna, S; Yamuy, J
Brain Research null
Wiphawi Hipkaeo et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(4), 479-490 (2004-03-23)
We studied the expression and localization of JunD, a component of the transcription factor activator protein-1 (AP-1), in the mouse submandibular gland with immunoblotting and immunohistochemistry. In adult mice, all seven Jun and Fos family members constituting the AP-1 complex
The role of LAT in increased CD8+ T cell exhaustion in trigeminal ganglia of mice latently infected with herpes simplex virus 1.
Allen, SJ; Hamrah, P; Gate, D; Mott, KR; Mantopoulos, D; Zheng, L; Town, T; Jones et al.
Journal of virology null
Sravya Kurapati et al.
Journal of virology, 91(17) (2017-06-24)
Mechanisms of neuronal infection by varicella-zoster virus (VZV) have been challenging to study due to the relatively strict human tropism of the virus and the paucity of tractable experimental models. Cellular mitogen-activated protein kinases (MAPKs) have been shown to play
Nicotinic alpha 7 receptor clusters on hippocampal GABAergic neurons: regulation by synaptic activity and neurotrophins.
Kawai, Hideki, et al.
The Journal of Neuroscience, 22, 7903-7912 (2002)

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