AB1528
Anti-Nerve Growth Factor-β Antibody
serum, Chemicon®
Sinónimos:
NGFbeta
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
sheep
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human, chicken, mouse
should not react with
bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NGF(4803)
Specificity
Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human NT4 and BDNF and approximately 2% for NT3 by ELISA.
Immunogen
Mouse nerve growth factor-beta purified from salivary glands.
Application
Detect Nerve Growth Factor-β using this Anti-Nerve Growth Factor-β Antibody validated for use in ELISA, WB, IH.
Immunohistochemistry at 1:500-1:5,000 (see suggested protocol).
Immunoblotting at 1:500-1:5,000.
ELISA at 1:500-1:5,000.
Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.
Use neat for in vivo animal studies (5-10 μL/g body weight).
Optimal working dilutions must be determined by end user.
Immunoblotting at 1:500-1:5,000.
ELISA at 1:500-1:5,000.
Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.
Use neat for in vivo animal studies (5-10 μL/g body weight).
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
Physical form
Whole sheep antisera. Lyophilized. Reconstitute with 250 μL of sterile distilled water.
Storage and Stability
Maintain at -20°C to -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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D Joelson et al.
Microscopy research and technique, 41(3), 246-262 (1998-05-30)
The distribution of immunoreactivity for the ionotropic N-methyl-D-aspartate (NMDA) receptor subunits was mapped in the cochlear nucleus of postnatal day (P) 7, P14, P21, and P28 gerbils. Frozen sections and serial plastic sections of tissue were incubated with antibodies to
Daniel Aridgides et al.
PloS one, 8(2), e57450-e57450 (2013-02-26)
Cardiomyocytes express neurotrophin receptor TrkA that promotes survival following nerve growth factor (NGF) ligation. Whether TrkA also resides in cardiac fibroblasts (CFs) and underlies cardioprotection is unknown. To test whether CFs express TrkA that conveys paracrine signals to neighbor cardiomyocytes
X F Zhou et al.
Journal of neuroscience methods, 54(1), 95-102 (1994-09-01)
Nerve growth factor (NGF) is a survival factor required by a number of neuronal populations including most post-ganglionic sympathetic neurones. NGF has been detected and quantified in many tissues but there is little information regarding its cellular localization. Although it
I K Abrahamson et al.
Journal of neurocytology, 16(3), 417-422 (1987-06-01)
Immunohistochemistry has been used to demonstrate the presence of nerve growth factor (NGF)-like immunoreactivity in normal and sectioned mouse sciatic nerves. In normal nerves, immunoreactive material was not visible unless a silk ligature had previously been applied to constrict the
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