324676

Elastase, Pseudomonas aeruginosa

Elastase, Pseudomonas aeruginosa, is a native metallopeptidase that hydrolyzes insoluble elastin. Shares a significant sequence homology with thermolysin.

• Sinónimos: Pseudolysin
• Comisión internacional de enzimas: 3.4.24.26 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.77

Propiedades

• Quality Level: 200
• assay: ≥90% (SDS-PAGE)
• form: lyophilized
• specific activity: ≥250 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: aqueous buffer: 1 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Descripción

General description

Native elastase from Pseudomonas aeruginosa. A metallopeptidase that hydrolyzes insoluble elastin. Shares a significant sequence homology with thermolysin. Pseudomonas aeruginosa elastase has been implicated in the reinforcement of the inflammation process in cases of infection with the bacterium.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µg insoluble elastin per h at 37°C.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 2 months at -20°C.

Other Notes

Azghani, A.O., et al. 2000. Lung178, 181.
Kon, Y., et al. 1999. FEMS Immunol. Med. Microbiol. 25, 313.
Braun, P., et al. 1998. J. Bacteriol. 180, 3467.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable