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Key Documents

21-170

Sigma-Aldrich

TOPflash (TCF Reporter Plasmid)

Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame.

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About This Item

UNSPSC Code:
41106617
eCl@ss:
34360190
NACRES:
NA.41

Quality Level

manufacturer/tradename

Upstate®

technique(s)

transfection: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

General description

TOPflash is a β-catenin-responsive firefly luciferase reporter plasmid.

Application

TOPflash (TCF Reporter Plasmid) has been used for transfection of cultured cells in TOP flash reporter gene assay.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jia-Hao Xiao et al.
The Journal of biological chemistry, 278(32), 29954-29962 (2003-05-29)
Beta-catenin is a component of stable cell adherent complexes whereas its free form functions as a transcription factor that regulate genes involved in oncogenesis and metastasis. Free beta-catenin is eliminated by two adenomatous polyposis coli (APC)-dependent proteasomal degradation pathways regulated
Species differences in kidney necrosis and DNA damage, distribution and glutathione-dependent metabolism of 1,2-dibromo-3-chloropropane (DBCP).
E J S?derlund,M L?g,J A Holme,G Brunborg,J G Omichinski,J E Dahl,S D Nelson,E Dybing
Pharmacology & Toxicology null
Haijun Zhao et al.
Oncology letters, 21(6), 472-472 (2021-04-29)
Long non-coding (lnc) RNAs have emerged as important regulators of cancer development and progression. Several lncRNAs have been reported to be associated with prostate cancer (PCa); however, the involvement of lncRNA SNHG17 in PCa remains unclear. In the present study
Sergio Roman-Roman et al.
The Journal of biological chemistry, 279(7), 5725-5733 (2003-11-25)
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Ji Young Choi et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(11), 2059-2070 (2018-07-13)
Patients with Hutchinson-Gilford progeria syndrome (HGPS) have low bone mass and an atypical skeletal geometry that manifests in a high risk of fractures. Using both in vitro and in vivo models of HGPS, we demonstrate that defects in the canonical

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