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1.25055

Millipore

Fractogel® EMD TMAE Hicap (M)

MiniChrom Column Fractogel® TMAE Hicap (M), volume 1 mL, prepacked column

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MiniChrom Column Fractogel® TMAE Hicap (M), 1 ml

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About This Item

UNSPSC Code:
41115711

ligand

(Trimethylammoniumethyl)

Quality Level

100

material

polypropylene housing

product line

Fractogel®

form

prepacked column

parameter

≤8 bar pressure
~180 cm/hr max. flow rate

column I.D. × L

8 mm × 20 mm

volume

1 mL

matrix active group

Methacrylate

mean particle size

40-90 μm

capacity

~180 mg, BSA binding capacity

application(s)

mAb
recombinant protein
vaccine development

separation technique

anion exchange

storage temp.

15-25°C

General description

8 x 20 mm

Features and Benefits

MiniChrom Column Fractogel® TMAE Hicap (M), 1 ml enables users to:

  • Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
  • Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior of Fractogel® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Analysis Note

Appearance: passes test

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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