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Key Documents

07-539

Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys 4) Antibody

Upstate®, from rabbit

Sinónimos:

H3K4Ac, Histone H3 (acetyl K4)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

acetylation (Lys 4)

Gene Information

human ... H3F3B(3021)

Specificity

Broad species cross reactivity is expected
Histone H3 when acetylated on Lys4

Immunogen

KLH-conjugated, synthetic peptide containing the sequence ...RT[AcK]Q... in which [AcK] corresponds to acetyl lysine 4 of human histone H3

Application

Suggested dilutions:
Western Blot: 1:1000 - 1:10,000
Anti-acetyl-Histone H3 (Lys 4) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys 4) also known as H3K4Ac, Histone H3 (acetyl K4) & has been validated in Mplex, WB, PIA, ChIP, ChIP-seq, DB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Routinely evaluated by immunoblot.

Target description

Mr ~17kDa

Physical form

100μl of immunoaffinity purified rabbit IgG in a buffer with 0.1M Tris-glycine, pH 7.4 and 0.15M NaCl, and containing 0.05% sodium azide and 30% glycerol
ImmunoAffinity Purified

Storage and Stability

1 year at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Federico Forneris et al.
The Journal of biological chemistry, 281(46), 35289-35295 (2006-09-22)
Human lysine-specific demethylase (LSD1) is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys4 of histone H3 (H3-K4). We used a combination of in vivo and in vitro experiments to characterize the substrate specificity and recognition
Sarah Javaid et al.
Cell reports, 5(6), 1679-1689 (2013-12-24)
Epithelial-mesenchymal transition (EMT) is thought to contribute to cancer metastasis, but its underlying mechanisms are not well understood. To define early steps in this cellular transformation, we analyzed human mammary epithelial cells with tightly regulated expression of Snail-1, a master
Flore Beurton et al.
Nucleic acids research, 47(21), 11164-11180 (2019-10-12)
The CFP1 CXXC zinc finger protein targets the SET1/COMPASS complex to non-methylated CpG rich promoters to implement tri-methylation of histone H3 Lys4 (H3K4me3). Although H3K4me3 is widely associated with gene expression, the effects of CFP1 loss vary, suggesting additional chromatin
Oral administration of the pimelic diphenylamide HDAC inhibitor HDACi 4b is unsuitable for chronic inhibition of HDAC activity in the CNS in vivo.
Beconi, M; Aziz, O; Matthews, K; Moumne, L; O'Connell, C; Yates, D; Clifton, S; Pett et al.
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Anatomical record (Hoboken, N.J. : 2007), 298(8), 1431-1439 (2015-05-09)
IL-4 activates STAT6 and causes the subsequent up-regulation of Ig heavy chain germline Igε via chromatin remodeling involved in B lymphocytes development. STAT6 acts as a molecular switch to regulate the higher-order chromatin remodeling via dynamically orchestrating co-activators (CBP/Tudor-SN) and

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