07-1585

Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402)

from rabbit, purified by affinity chromatography

• Sinónimos: Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• packaging: antibody small pack of 25 μg
• technique(s): western blot: suitable
• NCBI accession no.: NP_001521
• UniProt accession no.: Q16665
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... HIF1A(3091)

Descripción

General description

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with HIF-1β to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.

Specificity

This antibody recognizes HIF-1-alpha at the ODD region.

Immunogen

Epitope: ODD region

KLH-conjugated linear peptide corresponding to the ODD region of human HIF-1-alpha.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402) (Rabbit Polyclonal Antibody) validated in WB to detect HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha.

Quality

Evaluated by Western Blot in FeCl2 treated HIF-1-alpha recombinant proteins.

Western Blot Analysis: 1 µg/mL of this antibody detected HIF-1-alpha on 10 µg of FeCl2 treated HIF-1-alpha recombinant proteins.

Target description

~120 kDa observed using Gal4-tagged recombinant protein. 92 kDa calculated for native protein.

Linkage

Replaces: 04-1006

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
FeCl2 treated HIF-1-alpha recombinant proteins

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.