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Merck
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Key Documents

06-270

Sigma-Aldrich

Anti-Giα3 Antibody

serum, Upstate®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

hamster, mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GNAI3(2773)

Specificity

Recognizes Giα3 and Goα; low cross-reactivity with other G-proteins.

Immunogen

peptide corresponding to the C-terminal region of Giα3 (KNNLKECGLY)

Application

Detect Giα3 using this Anti-Giα3 Antibody validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Quality

routinely evaluated by immunoblot on rat hippocampus membrane preparation, solubilized rat and mouse brain membranes or mouse 3T3/A31 cell lysates

Target description

40 kDa

Physical form

Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Widely expressed

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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G(o)-protein alpha-subunits activate mitogen-activated protein kinase via a novel protein kinase C-dependent mechanism.
van Biesen, T, et al.
The Journal of Biological Chemistry, 271, 1266-1269 (1996)
J M Sands et al.
The Journal of clinical investigation, 99(6), 1399-1405 (1997-03-15)
During antidiuresis, increases in vasopressin (AVP)-elicited osmotic water permeability in the terminal inner medullary collecting duct (tIMCD) raise luminal calcium concentrations to levels (> or = 5 mM) above those associated with the formation of calcium-containing precipitates in the urine.
Disulfide bonds in the extracellular calcium-polyvalent cation-sensing receptor correlate with dimer formation and its response to divalent cations in vitro
Ward, D. T., et al
The Journal of Biological Chemistry, 273, 14476-14483 (1998)
P Barrett et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(11), 1619-1628 (2000-08-06)
Mimicking short photoperiod melatonin signals (16 h exposure) on primary cell cultures of melatonin target cells of the ovine pars tuberalis (PT) results in an enhanced cAMP response to forskolin stimulation relative to untreated cells, a phenomenon termed sensitization. The
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(2), 702-715 (2015-10-16)
Depletion of sarcoplasmic reticulum (SR) Ca(2+) stores activates store-operated channels (SOCs) composed of canonical transient receptor potential (TRPC) 1 proteins in vascular smooth muscle cells (VSMCs), which contribute to important cellular functions. We have previously shown that PKC is obligatory

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