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Key Documents

06-1304

Sigma-Aldrich

Anti-SETD8 (hPR-SET7) Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

Histone-lysine N-methyltransferase SETD8

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SETD8(387893)

General description

N-lysine methyltransferase KMT5A (UniProt: Q9NQR1; also known as EC: 2.1.1.43, H4-K20-HMTase KMT5A, Histone-lysine N-methyltransferase KMT5A, Lysine N-methyltransferase 5A, Lysine-specific methylase 5A, PR/SET domain-containing protein 07, PR-Set7, PR/SET07, SET domain-containing protein 8) is encoded by the KMT5A (also known as PRSET7, SET07, SET8, SETD8) gene (Gene ID: 387893) in human. SETD8 (hPR-SET7) is a histone methyltransferase that adds a single methyl group to lysine 20 of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. SETD8 is not detected during the G1 phase. It is first detected during S through G2 phases, and peaks during mitosis. It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis and is also involved in chromosome condensation and proper cytokinesis. It is also reported to mediate mono-methylation of p53/TP53 at lysine 382, leading to repress p53/TP53-target genes. SETD8 has a SET domain in the amino acids 257-378 and this region contains the active site of enzymatic activity. Sequences upstream and downstream of SET domain are also required for its methyltransferase activity. Two isoforms of SETD8 have been described that are produced by alternative splicing.

Specificity

This rabbit polyclonal antibody detects N-lysine methyltransferase KMT5A (PR-SET7).

Immunogen

GST-tagged recombinant protein corresponding to human SETD8 (hPR-SET7).

Application

Anti-SETD8 (hPR-SET7) Antibody is a Rabbit Polyclonal Antibody for detection of SETD8 (hPR-SET7) also known as Histone-lysine N-methyltransferase SETD8 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected SETD8 (hPR-SET7) in HeLa cell nucleaar extract.

Target description

~43 kDa

Linkage

Replaces: 07-316

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.

Storage and Stability

Stable at 2-8°C for 1 year from date of receipt.

Analysis Note

Control
HeLa nuclear extract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Chieh-Tien Shih et al.
Aging cell, 16(4), 797-813 (2017-05-18)
Cellular senescence is a permanent proliferative arrest triggered by genome instability or aberrant growth stresses, acting as a protective or even tumor-suppressive mechanism. While several key aspects of gene regulation have been known to program this cessation of cell growth
Stine Jørgensen et al.
The Journal of cell biology, 192(1), 43-54 (2011-01-12)
The eukaryotic cell cycle is regulated by multiple ubiquitin-mediated events, such as the timely destruction of cyclins and replication licensing factors. The histone H4 methyltransferase SET8 (Pr-Set7) is required for chromosome compaction in mitosis and for maintenance of genome integrity.
Giulia Saredi et al.
Nature, 534(7609), 714-718 (2016-06-25)
After DNA replication, chromosomal processes including DNA repair and transcription take place in the context of sister chromatids. While cell cycle regulation can guide these processes globally, mechanisms to distinguish pre- and post-replicative states locally remain unknown. Here we reveal
Hang Gyeong Chin et al.
The Journal of biological chemistry, 295(14), 4748-4759 (2020-03-01)
Microtubules are cytoskeletal structures critical for mitosis, cell motility, and protein and organelle transport and are a validated target for anticancer drugs. However, how tubulins are regulated and recruited to support these distinct cellular processes is incompletely understood. Posttranslational modifications

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