03-0191-00

SMC^® Human IFN-β1 High Sensitivity Immunoassay Kit

• Sinónimos: Interferon β-1 immunoassay kit, Interferon beta-1 Single Molecule Counting Kit, Interferon beta-1 immunoassay kit, Interferon beta-1 ultra-sensitive immunoassay
• UNSPSC Code: 12161503
• NACRES: NA.84

Propiedades

• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.15 pg/mL; (lower limit of quantification); inter-assay cv: 13%; intra-assay cv: 6%
• technique(s): ELISA: suitable; single molecule counting: suitable
• relevant disease(s): infectious diseases; autoimmune diseases; neurodegenerative disorders
• storage temp.: 2-8°C

Descripción

General description

A member of the type I interferon family of glycoprotein second messengers, human Interferon Beta (IFN-ß1) has been associated with antiviral activity as a secreted factor from infected cells that mediates innate immunity in neighboring cells. In addition, IFN-β1 has also been utilized as treatment for Multiple Sclerosis (MS) due to its ability to ameliorate inflammation in the central nervous system in this disease.

The SMC^® Human IFN-β1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO^® immunoassay systems detect analytes using Single Molecule Counting (SMC^®) technology.

Specificity

Cross Reactivity:
Cross-reactivity to the following analytes were tested with the following results:

• human IFN-α2 – 0.2%
• human IFN-γ – 0.3%
• human IL-29 (IFN-λ1) – 0.2%
• human IL-28A (IFN-λ2) – 0.2%
• human IL-28B (IFN-λ3) – 0.3%
• human IFN-ω – 0.2%

Accuracy:

• Minimum of 5 serum samples spiked with average recovery of 88% with a range of 78-100%
• Minimum of 5 plasma samples spiked with average recovery of 93% with a range of 75-113%

Application

Research Category:

• Inflammation & Immunology
• Immune Response
• Infectious Diseases
• Signaling
• Neuroscience

Research Sub-Category:

• Inflammatory Disease
• Immune Response
• Autoimmune disease
• Neurodegenerative Diseases
• Neuroregenerative Medicine
• Virology
• Infectious Diseases - Viral
• Immunological Signaling

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human IFN-β1 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-β1 in human serum and plasma.

Features and Benefits

This new streamline kit has the following features including:

• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Packaging

96 well plate

Components

• Assay Buffer
• IFN-β1 Coated Beads
• Standard Diluent
• IFN-β1 Detection Antibody
• IFN-β1 Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects