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906549

Sigma-Aldrich

SLAM-VproR-13CH3 Methyl Labeling Kit

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About This Item

UNSPSC Code:
12352200

technique(s)

bio NMR: suitable

Quality Level

200

shipped in

dry ice

storage temp.

−70°C

Categorías relacionadas

General description

SLAM-VproS-13CH3 Kit has13CH3 isotopomer precursor and contains protocol instructions for creation of isotopically-labeled proteins.

Application

For detection of long-range nOes or for the NMR study of large proteins
High incorporation of 13CH3 isotopomer of methyl groups in valine residues without detectable scrambling is achieved using SLAM-VproS-13CH3 kit. This kit has been tested with protein isotopic labeling in E. coli. It enables investigation of supramolecular complex upto 1 MDa in solid-state NMR spectroscopy studies.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Storage Class

11 - Combustible Solids

Certificados de análisis (COA)

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Stereospecific isotopic labeling of methyl groups for NMR spectroscopic studies of high-molecular-weight proteins.
Pierre Gans et al.
Angewandte Chemie (International ed. in English), 49(11), 1958-1962 (2010-02-17)
Rime Kerfah et al.
Current opinion in structural biology, 32, 113-122 (2015-04-17)
Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

Artículos

Methyl Isotope-Labeling of Proteins from NMR-Bio

Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.

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