ZRB2968
Anti-Homer 1 Antibody, clone 1F5 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
SYN47
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1F5, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 40.28 kDa
observed mol wt ~51 kDa
purified by
using Protein A
species reactivity
human, rat, mouse
species reactivity (predicted by homology)
bovine, monkey, avian, canine, (Whale), horse
packaging
antibody small pack of 25
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... HOMER1(9456)
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1F5 is a ZooMAb® rabbit recombinant monoclonal antibody that detects Homer-1. It targets an epitope within 17 amino acids from the C-terminal region of human Homer-1.
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of Homer-1.
Application
Quality Control Testing
Evaluated by Western Blotting in Neuro-2a cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Homer-1 in Neuro-2a cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Homer-1 in lysates from SH-SY5Y cells and Human brain tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Homer-1 in Rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Homer-1 in SH-SY5Y cells.
Affinity Binding Assay: A representative lot of this antibody bound Homer-1 peptide with a KD of 1.0 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Neuro-2a cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Homer-1 in Neuro-2a cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Homer-1 in lysates from SH-SY5Y cells and Human brain tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Homer-1 in Rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Homer-1 in SH-SY5Y cells.
Affinity Binding Assay: A representative lot of this antibody bound Homer-1 peptide with a KD of 1.0 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Homer protein homolog 1 (UniProt: Q86YM7; also known as Homer-1) is encoded by the HOMER1 (also known as SYN47) gene (Gene ID: 9456) in human. Homer protein homolog 1 (Homer-1) is a scaffolding protein involved in intracellular signaling pathways and neuronal function. It plays a critical role in receptor clustering, cell signaling modulation, and synaptic plasticity. Homer-1 contains an N-terminal coiled-coil region that allows for the formation of homo- and heteromeric interactions with other proteins, while the C-terminal domain binds to various target proteins involved in synaptic signaling. Homer-1 interacts with several proteins such as metabotropic glutamate receptors (mGluRs), inositol trisphosphate receptors (IP3Rs), and Shank proteins. Homer-1 is widely expressed in various tissues, but it is highly enriched in the brain, particularly in regions associated with synaptic transmission, such as the cortex, hippocampus, and cerebellum. Dysregulation of Homer-1 expression and function has been implicated in various neurological disorders, including schizophrenia, Alzheimer′s disease, epilepsy, and drug addiction. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Luo, P., et al., (2012) Neurochem. Int. 61, 731 738)
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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