ZRB2222

Anti-FLT4 Antibody, clone 2F4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Anti-TIM-3 Antibody, clone RMT3-23 ZooMAb^® Rat Monoclonal, EC:2.7.10.1, Fms-like tyrosine kinase 4, Tyrosine-protein kinase receptor FLT4, Vascular endothelial growth factor receptor 3
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2F4, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 152.76 kDa; observed mol wt ~140 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_891555
• UniProt accession no.: P35916
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... FLT4(2324)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2F4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects FLT4/VEGFR 3. It targets an epitope within 26 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 26 amino acids from the extracellular domain within the N-terminal half of human FLT4/VEGFR3.

Application

Quality Control Testing

Evaluated by Western Blotting with His-tagged recombinant extracellular domain of human FLT4.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged recombinant extracellular domain of human FLT4/VEGFR3

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FLT4/VEGFR3 in bEnd.3 cells.

Affinity Binding Assay:: A representative lot of this antibody bound FLT4 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected His-tagged recombinant extracellular domain of human FLT4/VEGFR3.

Flow Cytometry Analysis: A representative lot detected FLT4/VEGFR3 in one million Human umbilical vein endothelial cells (HUVEC).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Vascular endothelial growth factor receptor 3 (UniProt: P35916; also known as EC:2.7.10.1, VEGFR-3, Fms-like tyrosine kinase 4, FLT-4, Tyrosine-protein kinase receptor FLT4) is encoded by the FLT4 (also known as VEGFR3) gene (Gene ID: 2324) in human. FLT4 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-775), a transmembrane domain (aa 776-796), and cytoplasmic domain (aa 797-1363). It is expressed in endothelial cells and is also detected in fetal spleen, lung, and brain. In adults, it is detected in liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. FLT4 has tyrosine-protein kinase activity and acts as a receptor for VEGFC and VEGFD. It plays a role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Its secreted fo

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable