ZMS1158
Anti-Collagen Type VII Antibody, clone NP32 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
LC collagen, Long-chain collagen
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
NP32, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 295.22 kDa
observed mol wt ~300 kDa
purified by
using protein G
species reactivity
human
species reactivity (predicted by homology)
bovine
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... COL7A1(1294)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone NP32 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Collagen alpha-1(VII) chain. It targets an epitope within the NC-1 domain from the N-terminal half.
Immunogen
Type VII collagen from Human chorioamniotic membranes.
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Collagen Type VII in HeLa cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Collagen Type VII in HeLa cells.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Collagen Type VII in human frozen skin tissue sections.
Immunohistochemistry Applications: A representative lot detected Collagen Type VII in human skin tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Collagen Type VII in HeLa cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Collagen Type VII in HeLa cells.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Collagen Type VII in human frozen skin tissue sections.
Immunohistochemistry Applications: A representative lot detected Collagen Type VII in human skin tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Collagen alpha-1(VII) chain (UniProt: Q02388; also known as Long-chain collagen, LC collagen) is encoded by the COL7A1 gene (Gene ID: 1294) in human. Collagen is the most abundant protein in the body that is predominantly synthesized by fibroblasts. However, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagens with at least 38 distinct polypeptide chains. Collagen type VII is a homotrimeric protein that is primarily synthesized by keratinocytes and skin fibroblasts. It is composed of three identical alpha chains and its distribution is restricted to stratified squamous epithelial basement membrane. It contributes to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins. Type VII collagen contains a non-collagenous domain 1 (NC1), a central collagenous domain, and a non-collagenous domain 2 (NC2) that catalyze the anchoring fibril assembly. Mutations in COL7A1 gene have been linked to epidermolysis bullosa dystrophica, a blistering skin disease characterized by tissue separation that occurs below the dermal-epidermal basement membrane at the level of the anchoring fibrils. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Supp, DM., et al. (2019). Cell Transp. 28(9-10); 1242-1256; Heagerty, AHM., et al. (1986). J. Invest. Dermatol. 86(5); 603-605).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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