T9701

TRAIL human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE and HPLC), lyophilized powder

• Synonym(s): Apo2 Ligand, Apo2L, TL2, TNF-related apoptosis-inducing ligand
• CAS Number: 2244730-93-4
• MDL number: MFCD01867158
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• description: extracellular domain
• Assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• mol wt: 19.6 kDa; predicted mol wt ~21 kDa
• technique(s): immunofluorescence: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P50591
• storage temp.: −20°C
• Gene Information: human ... TNFSF10(8743)

Description

Application

Human TRAIL can be used as a selection agent for treating human cells for phenotypic enrichments. The product can also be used in chemical transfection for identifying Small RNA Modulators of TRAIL-Induced Apoptosis Pathway by utilizing libraries of synthetic small interfering RNA (siRNA) and microRNAs (miRNA).

TRAIL human has been used in immunofluorescence analyses, to study the delocalization of melanoma antigen-D2 in the nucleoplasm after a cellular stress.

Biochem/physiol Actions

TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. The product present in both soluble as well as membrane-bound form, can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induce apoptotic cell death in TRAIL-sensitive U343MG cells.

TRAIL is a cytokine with anti-inflammatory, and antitumor activity. It is expressed in various cells including immune cells, vascular cells and adipocytes. TRAIL is associated with vascular endothelial cell protection.

Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.

Physical form

Lyophilized from phosphate buffered saline

Analysis Note

The biological activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive LN-18 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable