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Key Documents

T3285

Sigma-Aldrich

TNM-FH insect medium

With ʟ-glutamine and sodium bicarbonate,liquid, sterile-filtered, suitable for insect cell culture

Synonym(s):

supplemented Grace′s medium

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

product name

TNM-FH insect medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | insect: suitable

components

NaHCO3: 0.35 g/L
L-glutamine: 0.6 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

Several modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species. 

Application

TNM-FH insect medium has been used to culture Sf9 insect cells.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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