SRP6383

CD319/SLAMF7/CRACC human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): 19A, CS1, FOAP-12
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 23.2 kDa; observed mol wt 33-48 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 23 is the predicted N-terminus.)
• packaging: pkg of 10 μg; pkg of 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: Q9NQ25
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... SLAMF7(57823)

Description

General description

Research area: IMMUNO AND CKS

SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. SLAM F7 (signaling lymphocytic activation molecule F7) receptors are expressed on immune cells such as activated T cells, most B cells, antibody-producing plasma cells and natural killer cells and also on myeloid cells. The SLAMF7 gene is mapped to human chromosome 1q23.3.

Biochem/physiol Actions

SLAMF7 (signaling lymphocytic activation molecule F7) is considered as a self-ligand which identifies another SLAMF7 expressed on the cell. Apart from immune cells, SLAMF7 is highly expressed in multiple myeloma cells.Isoform 1 of SLAMF7 mediates natural killer (NK) cell activation through a SH2 domain containing 1A (SH2D1A)-independent extracellular signal-regulated extracellular signal-regulated kinases (ERK) mediated pathway. It may play a role in lymphocyte adhesion.Isoform 3 of SLAMF7 does not mediate any NK cell activation.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable