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Key Documents

SRP6263

Sigma-Aldrich

PLGF/PIGF2/PGF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

PGFL, SHGC-10760

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 20.2 kDa
observed mol wt 28-33 kDa (DTT-reduced. Protein migrates due to glycosylation. Arg 29 is the predicted N-terminus.)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P49763

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PLGF(5228)

General description

Placental growth factor (PLGF) exists in two isoforms namely PLGF1 and PLGF2. This protein is a homodimeric glycoprotein with molecular weight of 46–50kDa and is a member of the VEGF (vascular endothelial growth factor) subfamily. PLGF is encoded by the gene mapped to human chromosome 14q24-q31 regions. PLGF is expressed mainly in the placental trophoblast during pregnancy and is also present in various other tissues such as the villous trophoblast.

Biochem/physiol Actions

PLGF might enhance the production of peripheral blood mononuclear cell-vascular endothelial growth factor (PBMC-VEGF). Consequently, PlGF might facilitate VEGF production in the inflammatory joint. Soluble fms related tyrosine kinase 1 (Flt-1), which is considered to be an anti-angiogenic factor interacts with and inhibit the activity of PLGF. Therefore, PLGF level is lowered and the Flt-1 level is increased in placental tissue of women with pre-eclampsia (PE). PLGF is associated with the pathogenesis of rheumatoid arthritis (RA) and various other arthropathies. PLGF acts as a ligand for VEGFR-1 and is implicated in various physiological functions such as tumor angiogenesis, wound healing and hematopoietic progenitors recruitment.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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PlGF blockade does not inhibit angiogenesis during primary tumor growth.
Bais C
Cell, 141(1), 166-177 (2010)
Role of placenta growth factor (PIGF) in human extravillous trophoblast proliferation, migration and invasiveness.
Athanassiades A and Lala PK
Placenta, 19(7), 465-473 (1998)
Placenta growth factor (PlGF) induces vascular endothelial growth factor (VEGF) secretion from mononuclear cells and is co-expressed with VEGF in synovial fluid.
Bottomley MJ
Clinical and Experimental Immunology, 119(1), 182-188 (2000)
Effect of antihypertensive therapy with alpha methyldopa on levels of angiogenic factors in pregnancies with hypertensive disorders.
Khalil A
PLoS ONE, 3(7), e2766-e2766 (2008)
Assignment of vascular endothelial growth factor (VEGF) and placenta growth factor (PLGF) genes to human chromosome 6p12-p21 and 14q24-q31 regions, respectively.
M G Mattei et al.
Genomics, 32(1), 168-169 (1996-02-15)

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