SRP6257

ADIPOQ/Adiponectin/ACRP30 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

• Synonym(s): ACDC, ACRP30, ADIPOQ, APM1, Adiponectin, GBP28
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥92% (SDS-PAGE)
• form: lyophilized powder
• mol wt: calculated mol wt 25.4 kDa; observed mol wt 28-33 kDa (DTT-reduced. Protein migrates due to glycosylation. Glu 19 is the predicted N-terminus.)
• packaging: pkg of 10 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: Q15848
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... ADIPOQ(9370)

Description

General description

Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. The protein has a carboxyl-terminal globular domain and an amino-terminal collagen domain. The gene is mapped to human chromosome 3q27.

Biochem/physiol Actions

Adiponectin (ADIPOQ) has anti-atherosclerotic and anti-inflammatory properties. ADIPOQ has a role in physiological processes such as energy homeostasis and obesity. It exhibits anti-diabetic action by interacting with adiponectin receptors, AdipoR1 and AdipoR2. Low levels of adiponectin are also linked with hypertension and dyslipidemia. Mutation in the gene encoding it is linked with risk for endometrial cancer.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable