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Key Documents

SRP3253

Sigma-Aldrich

SDF-1 beta from rat

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):

CXCL12, PBSF, Stromal-Cell Derived Factor-1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rat

recombinant

expressed in E. coli

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

100.0-200.0 ng/mL

mol wt

8.4 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

yellow-white

UniProt accession no.

Q9QZD1

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... CXCL12(24772)

General description

SDF-1 alpha and beta are stromal derived CXC chemokines, and signal through the CXCR4 receptor. SDF-1alpha and beta chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. Recombinant rat SDF-1b is an 8.4 kDa protein containing 72 amino acid residues.

Biochem/physiol Actions

SDF-1 alpha and beta are stromal derived CXC chemokines, and signal through the CXCR4 receptor. Recombinant rat SDF-1b is an 8.4 kDa protein containing 72 amino acid residues.

Sequence

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KM

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M D'Apuzzo et al.
European journal of immunology, 27(7), 1788-1793 (1997-07-01)
In the bone marrow, progenitor (pro-) and precursor (pre-) B cells depend on close contact with stromal cells for growth and maturation. Stromal cell-derived factor 1 (SDF-1), also known as pre-B cell growth-stimulating factor, is produced by bone marrow stromal
E Oberlin et al.
Nature, 382(6594), 833-835 (1996-08-29)
A putative chemokine receptor that we previously cloned and termed LESTR has recently been shown to function as a co-receptor (termed fusin) for lymphocyte-tropic HIV-1 strains. Cells expressing CD4 became permissive to infection with T-cell-line-adapted HIV-1 strains of the syncytium-inducing

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