SRP2014

RNA Polymerase II, p33 subunit human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(s): RPB3, RPB31, hRPB33, hsRPB3
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~33.5 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 350 μg/mL
• technique(s): western blot: suitable
• color: clear colorless
• NCBI accession no.: NM_032940
• UniProt accession no.: P19387
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... POLR2C(5432)

Description

Biochem/physiol Actions

hRPB3 (p33) is a highly conserved subunit shared by all three RNA polymerases. It has been shown to be in close contact to promoter DNA when pol II is recruited into the preinitiation complex. RPB3 has also been implicated in direct protein-protein contacts with transcription factor IIB, Rap30 subunit of transcription factor IIF, and gene-specific modulator proteins, such as the hepatitis B virus transactivator protein X or an inhibitor of pol II, RMP (RPB3-mediating protein). Therefore, RPB3 is facilitating the communication between the pol II core and a variety of basal and gene-specific transcription factors. In the pol II complex, RPB3 interacts with RPB5 and the RPB3–RPB5 interaction is intensified in the presence of three subunits, RPB7, RPB8 and RPB11. RPB3 also makes direct contacts with both RPB1 and RPB2, the two large subunits of the polymerase complex.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable