SAB4200843

Anti-BCOR antibody, Mouse monoclonal

clone BR-4, purified from hybridoma cell culture

• UNSPSC Code: 12352203

Properties

• antibody form: purified from hybridoma cell culture
• clone: BR-4
• form: liquid
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 2-4 μg/mL using human HeLa nuclear extract; immunofluorescence: 2-4 μg/mL using human HeLa cells
• isotype: IgG1
• UniProt accession no.: Q6W2J9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

BCOR encodes a ubiquitously expressed protein which is involved in BCL6-mediated transcriptional repression.1 Originally it was identified as a novel BCL6 corepressor via its interaction with the site-specific DNA-binding transcription factor BCL6, whose aberrant expression drives formation of diffuse large B-cell lymphomas.

Specificity

Monoclonal Anti-BCOR antibody specifically recognizes human BCOR protein.

Application

The antibody may be used in various immunochemical techniques including Immunoblotting (∼190 kDa) and Immunofluorescence.

Biochem/physiol Actions

NCOR and SMRT, was recently demonstrated to occupy the majority of its DNA binding sites independently from BCL6, reflecting its multiple interaction partners. BCOR also forms a Polycomb repressor complex 1 (PRC1)-like complex with PCGF1, KDM2B, RING1, SKP1, RYBP, and RNF2, and can bind to both class I and II histone deacetylases.1 BCOR is specifically and strongly expressed during embryonic development where it plays a critical role in transcriptional regulation, as BCOR mutations are associated with the X-linked inherited diseases Lenz microphthalmia and Oculofaciocardiodental syndrome (OFCD).

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable