RPROB

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• usage: sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
• Quality Level: 200
• technique(s): protein staining: suitable
• storage temp.: 2-8°C

Description

Application

R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.

Other Notes

Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.

Kit Components Only

• Solution A 500 mL
• Solution B 500 mL

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290 - H315 - H319
• Precautionary Statements: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• Storage Class Code: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 1