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R8781

Sigma-Aldrich

Rapamycin

from Streptomyces hygroscopicus, ≥95% (HPLC), solution, mTOR inhibitor

Synonym(s):

23,27-Epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine solution

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About This Item

Empirical Formula (Hill Notation):
C51H79NO13
Molecular Weight:
914.17
EC Number:
200-664-3
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Rapamycin, Ready Made Solution, 2.5 mg/mL in DMSO (2.74 mM), from Streptomyces hygroscopicus

biological source

Streptomyces hygroscopicus

Quality Level

300

vapor pressure

0.56 hPa ( 20 °C)

Assay

≥95% (HPLC)

form

solution

autoignition temp.

301 °C

concentration

2.5 mg/mL in DMSO (2.74 mM)

color

colorless to yellow

antibiotic activity spectrum

fungi
yeast

Mode of action

enzyme | inhibits

storage temp.

−20°C

Related Categories

General description

Rapamycin is a macrocyclic triene antibiotic possessing potent immunosuppressant and anticancer activity. It forms a complex with FKBP12 that binds to and inhibits the molecular target of rapamycin (mTOR). mTOR is a member of the phosphoinositide kinase-related kinase (PIKK) family that enhances cellular proliferation via the phosphoinositol 3-kinase/Akt signaling pathway. Inhibition of this pathway by rapamycin blocks downstream elements that result in cell cycle arrest in G1. The effectors of mTOR action include 4EBP1 and S6K1.
Rapamycin, an mTOR inhibitor, is derived from Streptomyces hygroscopicus. It can inhibit viral replication. This natural anti-fungal antibiotic is a proven anti-aging agent. Rapamycin significantly inhibits mTORC1 regardless of age.

Application

Rapamycin has been used:
  • as a supplement in mTeSR1 medium to culture induced pluripotent stem cell (iPSC) colonies for induction of autophagy signaling
  • to treat the Drosophila S2 cells at 65-70% confluence,
  • to measure protein synthesis
  • to stimulate autophagy in ovarian cancer cells

Packaging

200uL

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Keep container tightly closed in a dry and well-ventilated place. Store under inert gas. Is Hygroscopic.
Rapamycin has been expertly reviewed and recommended by the Chemical Probes Portal. For more information, please visit the Rapamycin probe summary on the Chemical Probes Portal website.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Yolanda T Chong et al.
Cell, 161(6), 1413-1424 (2015-06-06)
Proteomics has proved invaluable in generating large-scale quantitative data; however, the development of systems approaches for examining the proteome in vivo has lagged behind. To evaluate protein abundance and localization on a proteome scale, we exploited the yeast GFP-fusion collection
S Boridy et al.
Cell death & disease, 5, e1216-e1216 (2014-05-09)
Glioblastoma multiforme is a devastating disease of the central nervous system and, at present, no effective therapeutic interventions have been identified. Celastrol, a natural occurring triterpene, exhibits potent anti-tumor activity against gliomas in xenograft mouse models. In this study, we
Seokjo Kang et al.
Journal of Alzheimer's disease : JAD, 70(3), 667-680 (2019-07-01)
Increased levels of total tau (t-tau) and hyperphosphorylated tau (p-tau) proteins in the cerebrospinal fluid of Alzheimer's disease (AD) patients are well documented and strongly correlate with AD pathology. Recent studies have further shown that human tau can be released
H Sun et al.
British journal of cancer, 111(2), 281-291 (2014-06-20)
We investigated the biologic and pharmacologic activities of a chromosome region maintenance 1 (CRM1) inhibitor against human non-small cell lung cancer (NSCLC) cells both in vitro and in vivo. The in vitro and in vivo effects of a novel CRM1
A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
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