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MAK007

Sigma-Aldrich

Asparaginase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Asparaginase Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Asparaginase is an enzyme that catalyzes the hydrolysis of asparagine to aspartate. While asparaginase is synthesized by plants, microorganisms, and some animals, it does not occur naturally in humans. While most cells have the ability to synthesize asparagine, many hematopoietic cells do not and depend on exogenous asparagine for protein synthesis. Treatment of certain hematopoietic malignancies such as acute lymphoblastic leukemia (ALL) with asparaginase results in depletion of blood levels of asparagine resulting in cell cycle arrest and apoptosis. Asparaginase is also used in the food industry to reduce the formation of acrylamide in starchy and fried foods.

Suitability

Suitable for measuring asparaginase activity in biological samples

Principle

The Asparaginase Activity Assay Kit provides a simple and direct procedure for measuring Asparaginse activity in a variety of biological samples. Asparaginase activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate generated. One milliunit (mU) of Asparaginase is defined as the amount of enzyme that catalyzes the formation of 1.0 mmole of aspartate per minute at 25 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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An inhibitor of human asparagine synthetase suppresses proliferation of an L-asparaginase-resistant leukemia cell line.
Gutierrez J A, et al.
Chemistry and Biology, 13(1), 1339-1347 (2006)
Current applications and different approaches for microbial l-asparaginase production.
Cachumba J J M, et al.
Brazilian Journal of Microbiology, 47, 77-85 (2016)
Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
.Zhang J, et al.
Molecular Cell, 56(2), 205-218 (2014)
Semi-permeable coatings fabricated from comb-polymers efficiently protect proteins in vivo.
Liu M, et al.
Nature Communications, 5, 5526-5526 (2014)
Yalin Zhang et al.
The American journal of Chinese medicine, 47(5), 1133-1147 (2019-07-18)
Hyperuricemia is a metabolic disease of the kidney that results in decreased uric acid excretion. Here, we aimed to investigate the effects of ginsenosides and anserine on hyperuricemia and the expression of aquaporin (AQP) 1-4, which are indicators of renal

Articles

Enzymatic Asparaginase Activity Assay

This assay protocol is suitable for the colorimetric/fluorometric detection of Asparaginase activity in bacteria, cell and tissue culture supernatants, plant samples, biological samples, and food samples using the Asparaginase Activity Assay Kit (MAK007).

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