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M4434

Sigma-Aldrich

Anti-Mouse IgG2a (heavy chain specific) antibody produced in goat

affinity isolated antibody, lyophilized powder

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About This Item

MDL number:
MFCD02570704
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

technique(s)

Ouchterlony double diffusion: suitable

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
Anti-Mouse IgG2a (heavy chain specific) antibody recognizes mouse IgG2a when tested against purified mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM, myeloma proteins.

Immunogen

purified mouse IgG2a (myeloma protein).

Application

Anti-Mouse IgG2a (heavy chain specific) antibody is suitable for Ouchterlony double diffusion.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hiwa Karimi et al.
Reproductive toxicology (Elmsford, N.Y.), 69, 196-203 (2017-03-13)
A study using a mouse IVF model was conducted to examine the hypothesis that in vitro fertilization (IVF) treatment may lead to immune system alteration in the offspring. Phagocytic activity and lymphocyte proliferative responses to mitogen, alloantigen, and purified protein
Roselind S Lam et al.
Journal of immunology (Baltimore, Md. : 1950), 193(5), 2349-2362 (2014-07-30)
The role of the macrophage in the immunopathology of periodontitis has not been well defined. In this study, we show that intraoral inoculation of mice with Porphyromonas gingivalis resulted in infection, alveolar bone resorption, and a significant increase in F4/80(+)
S Hashira et al.
Pediatrics international : official journal of the Japan Pediatric Society, 42(4), 337-342 (2000-09-15)
Maternal immunoglobulin G (IgG), transferred across the placenta to the fetus during intrauterine life, is an important component of the neonatal immunological defence mechanisms against infection. There is controversy with respect to differences in placental transfer of the different IgG

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