L2542
LB Broth (Miller)
Liquid microbial growth medium
Synonym(s):
Luria Broth Miller, Luria-Bertani broth, Miller
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About This Item
UNSPSC Code:
41106200
NACRES:
NA.85
Recommended Products
grade
for molecular biology
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
pH
6.5-7.2
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
General description
Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.
Application
LBBroth (Miller) is suitable for:
- non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
- selective cultivation when appropriate antibiotics are added.
- the cultivation of E-coli cells for anti-bacterial assays.
Features and Benefits
Miller′s LB liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Components
10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
10 g/L NaCl
5 g/L Yeast Extract
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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