Skip to Content
Merck
All Photos(1)

Documents

H7411

Sigma-Aldrich

Anti-HA−FITC antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00803873
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

buffered aqueous solution

storage condition

protect from light

concentration

1 mg/mL

technique(s)

immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.
Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:
  • in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
  • in western blotting detection of spindle-associated proteins in Trypanosoma brucei
  • in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

Biochem/physiol Actions

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Aortic aneurysm generation in mice with targeted deletion of integrin-linked kinase in vascular smooth muscle cells
Shen D, et al.
Circulation Research, 109(6), 616-628 (2011)
Annalena La Porte et al.
Journal of virology, 90(21), 9889-9904 (2016-08-26)
INI1/hSNF5/SMARCB1/BAF47 is an HIV-specific integrase (IN)-binding protein that influences HIV-1 transcription and particle production. INI1 binds to SAP18 (Sin3a-associated protein, 18 kDa), and both INI1 and SAP18 are incorporated into HIV-1 virions. To determine the significance of INI1 and the
Faithful chromosome segregation in Trypanosoma brucei requires a cohort of divergent spindle-associated proteins with distinct functions
Zhou Q, et al.
Nucleic Acids Research, 46(16), 8216-8231 (2018)
Use of FITC-labeled influenza virus and flow cytometry to assess binding and internalization of virus by monocytes-macrophages and lymphocytes
Nichols JE, et al.
Archives of Virology, 130(3-4), 441-455 (1993)
Judith M Fonville et al.
PLoS pathogens, 11(10), e1005204-e1005204 (2015-10-07)
A high particle to infectivity ratio is a feature common to many RNA viruses, with ~90-99% of particles unable to initiate a productive infection under low multiplicity conditions. A recent publication by Brooke et al. revealed that, for influenza A
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service