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GE29-0510-21

HisTrap™ High Performance

Cytiva 29-0510-21, pack of 1 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

packaging

pack of 1 mL

manufacturer/tradename

Cytiva 29-0510-21

availability

not available in North America

parameter

flow rate
4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium)

capacity

≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.)
 ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

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General description

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Application

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

Features and Benefits

  • High binding capacity, at least 40 mg/ml chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Legal Information

HisTrap is a trademark of Cytiva

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Performing an isolation of recombinant protein complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Protocols

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service