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Key Documents

GE17-0050-01

Sephadex® G-75

Cytiva 17-0050-01, pack of 100 g

Synonym(s):

Molecular sieve

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About This Item

UNSPSC Code:
41115722
NACRES:
NA.56

feature

autoclavable In wet form (pH 7.0) at 120°C for 30 min

packaging

pack of 100 g

manufacturer/tradename

Cytiva 17-0050-01

parameter

flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
 flow rate

matrix

cross-linked dextran

particle size

40-120 μm (dry)
90-280 μm (wet)

working range

2-10

General description

Sephadex® G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Features and Benefits

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight
  • Highest exclusion limit, 80 000, in the Sephadex® G-range
  • Classic gel filtration medium.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Performing a Separation with Sephadex®

This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

Related Content

Separation Options with Sephadex®

Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service